Alphaamylase inhibition and antioxidative capacity of some. The comparison of two tannin extraction methods from. Plant extract as reducing agent in synthesis of metallic nanoparticles. Evaluation of superoxide radical scavenging capacity and. Pdf plant extract as reducing agent in synthesis of. Evaluation of antioxidant activity of medicinal plant extracts. Antioxidant properties of the methanol extracts of the leaves. Phytochemical analysis and antioxidants activities of. Iron reducing power was determined based on modified of oyaizu method 1986.
The results of reducing power activity showed that at 50. Evaluation of free radical scavenging power the ferric complexes reducing ability of the extracts by frap assay were measured at low ph. A yellow color indicated the presence of flavonoids. In vitro antioxidant activity of hydro alcoholic extract from the fruit. The antioxidant activity was investigated using the ferric reducing antioxidant power frap assay for plant extracts was investigated according to the method of oyaizu 1986 27. Solms, as a function of their concentration is shown in fig. Antioxidant potential of various extracts from ferula.
The reducing power of the plant extracts was determined by the method described by. The antioxidant activity of alcoholic and aqueous extracts was evaluated using 2, 2diphenyllpicrylhydrazyl dpph, 2, 2azinobis 3ethylbenzothiazoline6sulfonic acid abts and ferric reducing antioxidant power. Antioxidant activities of the extracts were evaluated by 2,2diphenyl1picrylhydrazyl dpph free radicalscavenging ability, trolox equivalent antioxidant capacity teac, and ferric reducing antioxidant power frap assays. The study of phenolic compounds antioxidant activity in. Antioxidant and antimicrobial activity of zingiberaceae. The reducing power of the extracts and standard are in the order. A simple, automated test measuring the ferric reducing ability of plasma, the frap assay, is presented as a novel method for assessing antioxidant power. Assessment of antioxidant activity of plant extracts by. Plants are rich source of free radical scavenging molecules such as vitamins, terpenoids, phenolic acids, lignins, stilbenes, tannins, flavanoids. The antioxidant activity was studied in some invitro antioxidant models like dpph radical scavenging activity, superoxide radical scavenging activity, ferric reducing power and hydrogen peroxide scavenging activity. Plant polyphenols play a vital role in conferring antioxidant property and, hence, the total polyphenol content tpc of the extract was estimated using the folinciocalteu method. Antioxidant activity and free radical scavenging capacity. A comparative analysis of antioxidant potentials of aqueous. Antioxidant activities of aqueous extracts of selected plants.
Evaluation antioxidant and antibacterial activities of n. Reducing power assay antioxidant capacity as per reducing power assay was measured according to a method reported by oyaizu 21. Effects of some plant extracts on the oxidative stability of. Extract, free radical, antioxidant activity, medicinal.
A strong reducing power was noted for methanol and acetone extracts of c. They have been extensively used in the treatment of various diseases including human cancer. Extract concentration providing 50% inhibition ic 50 was calculated from the plot of inhibition percentage against extract concentration. Different concentrations of the four extracts in 1 ml methanol were mixed with phosphate buffer 2. Radical scavenging and reducing power of salvia mirzayanii. Anchomanes difformis, dpph, phytochemicals, reducing power, total phenolics 115. The reducing power was increased with increasing amounts of extract. Jun 01, 2016 ferric reducing antioxidant power assay in plant extract article pdf available in bangladesh journal of pharmacology 1. Introduction one of the natural mechanisms used by all aerobic organisms, including humans. Free radicals scavenging activity and reducing power of two algerian sahara medicinal plants extracts. The reduction and stabilization of silver ions by combination of biomolecules such as proteins, amino acids. Pdf free radicals scavenging activity and reducing power. Frap ferric reducing ability of plasma assay and effect.
Dpph radical scavenging activity and reducing power of. The powder samples and methanol extract of 11 medicinal plants were subjected to analysis of proximate composition and measurement of antioxidant activity. Materials and methods plant collection six tonnes of fresh water hyacinth was collected from singanallur boat house, coimbatore, tamilnadu. Readings of the colored product ferrous tripyridyltriazine complex are noted at 593 nm. Free radicals scavenging activity and reducing power of two. Reducing power of eucalyptus oleosa leaf extracts and green. In this study, 18 species of five genus of zingiberaceae plants from taiwan area were collected and analyzed for their functional properties. The reducing powers of extracts were determined as per the reported method 15. Antioxidant properties of various solvent extracts of lemon. Ferric reducing power of the extract was also evaluated by oyaizu method. Dpph, ferric reducing power and nitric oxide as an antioxidant model. All plant extracts, except for tarragon, retarded peroxide formation at 80c the most effective being sage and sweet grass extracts fig. The in vitro antisickling and antioxidant effects of aqueous. The potassium ferricyanide reduction method is a widelyused method for evaluating the reducing power of plant extracts.
Reducing power the reducing power of meo was measured dissolved in various organic solvents using the method of chung et al25. Radicalscavenging activity and ferric reducing ability of. The ferric reducing ability of plasma frap as a measure of. Estimation of phytochemical content and antioxidant. The results suggest that nbutanol extract of the plant is very rich in antioxidant compounds worthy of further investigations. Effect of extraction solventtechnique on the antioxidant activity of selected medicinal plant extracts article pdf available in molecules 146. The reducing power activity of 70% ethanolic extract tubers of momordica tuberosa. Phytochemical screening and antioxidant activity of. A factor rotation using the varimax method was performed and two factor loadings were obtained that accounted for the total covariance of the plant extracts. One gram of dry plant material was homogenized with 20 ml of solvent solution acetoneultrapure waterglacial acetic acid, 70. Comparative analysis of the antioxidant activity of cassia. The antioxidant activity of grewia carpinifolia extract may be due to the high level of flavonoids and. Fruit, vegetable and plant extractions can be done using acidmethanol for e. Phytochemicals, antioxidant and antimicrobial potentials.
May 18, 2010 areca is a traditional chinese medicinal plant used to treat a variety of diseases. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. The noticeable of the reducing power activity and the antioxidant properties of this plant could serve as a significant indicator of the antioxidant potential. Reducing power of different extracts increased with the concentration of the extract. Dec 20, 2007 the rhizomes of the zingiberaceae family are a vegetable widely used in many asian countries, and their medicinal functions have been broadly discussed and accepted in many traditional recipes. The antioxidant capacities of areca flower extracts were evaluated by using superoxide radical scavenging activity and reducing power, total phenolic content and total flavonoid content were also detected. In vitro antioxidant and antimalarial activities of leaves. The ferric reducing antioxidant power frap mechanism is based on electron transfer rather than hydrogen atom transfer prior et al. Reducing power activity reducing power assay was determined according to the method of yildirim et al. The assays employed were ferric reducing antioxidant power, trolox equivalent antioxidant. This means that a repeated process will extract more or less the same amount in the solution, and the ratio cannot be whatever you may request or prefer.
Both extracts showed effective free radical scavenging activity. Preliminary phytochemical screening and invitro antioxidant activity of gymnema sylvestre r. Plant extract of 1, 10, 50 or 100 mg in 1 ml of ethanol was mixed with 2. Ascorbic acid solutions used as standard are prepared in the same way. Pdf free radicals scavenging activity and reducing power of two. Antibacterial efficacy of carica papaya leaves and stem. Comparison of in vitro antioxidant properties of extracts. Three different methods were used to test the antioxidant activity of the extract, including frap assay ferric reducing antioxidant potential, dpph radical scavenging assay 1,1diphenyl2picryl hydrazyl radical reducing power.
The reducing power was confirmed by the changes of yellow colour of the test solution to various shades of green and blue depending on the concentration of the plant extract. Free radicals scavenging activity and reducing power of two algerian sahara medicinal plants extracts abderrahim benslama and abdenassar harrar abstract the aim of this study is to evaluate antioxidant activity of aqueous aq. The use of plants as the production assembly of silver nanoparticles has drawn attention, because of its rapid, ecofriendly, nonpathogenic, economical protocol and providing a single step technique for the biosynthetic processes. Antioxidant and free radical scavenging activity of. After one hour at room temperature, the absorbance was measured at 420 nm. Different concentrations of methanolic extracts of leaf 200, 300, 400, 500 and 600. All the extracts tested exhibited the lower power reducing activity compared to catechin. Evaluation of antioxidant capacity of plant extracts by. Plantmediated green synthesis of iron nanoparticles. Natural antioxidants present in the plants scavenge harmful free radicals from our.
A variety of fruit, vegetable and plant samples, beverages as well as serum and plasma can be used with this assay. Inhibitory effect of selected medicinal plant extracts on phytopathogenic fungus fusarium oxysporum nectriaceae. In vitro antioxidant activity and free radical scavenging. May 16, 2016 therefore, the present study aims to determine and compare the antioxidant potential of aqueous leaf extracts of juniperus thurifera, juniperus phoenicea, juniperus oxycedrus, and tetraclinis articulata using dpph free radicalscavenging activity dpph, trolox equivalent antioxidant capacity teac, and ferric reducing antioxidant power frap. Original article comparison of abts, dpph, frap, and orac.
Aliquots of 1 ml of methanolic extract of each sample at 4 different concentrations. Standardized methods for the determination of antioxidant. Assay of the antioxidant content and reducing power of the plant extracts is a crucial point in phytochemistry, the food industry, and nutrition. Various concentrations of the extracts 500 l were mixed with 500 l of sodium phosphate buffer 200 mm, ph 6. Compared to aqueous extract, ethanolic extract has shown better activity.
Estimation of the total flavonoids in the plant extracts was carried out using the method of ordon ez et al. Frap values are obtained by comparing the absorbance change at 593 nm in test. Es2664025t3 synthesis of nanoparticles using plant and. Phytochemical analysis and antioxidants activities of aqueous. On the other hand, green synthesis of noble metal nanoparticles, such as gold by the aid of plant extract is dependent on the reducing power and antioxidant content of the extract. Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq. Materials and methods leaves of aloe vera, bacopa monniera, moringa oleifera and rhizome of zingiber officinale were collected from loni and adjoining areas, maharashtra. The reducing power of the extract and the standard drugs increased with an increase in concentration though the plant extract had higher antioxidant activity than the bht. A chloroform extract of cymbopogon citratus was screened to determine its free radical scavenging activities. The extract recovery in different solvents was expressed as percent of the plant sample dry matter.
The quality of the extracts prepared was assessed by determining their antioxidant activity using the ferric reducing antioxidant power frap method. Antibacterial efficacy of carica papaya leaves and stem bark extracts on clinical isolates of methicillin resistant staphylococcus aureus mrsa umar a1, nas fs2 and ali m3 1biological science unit, ahmadu bello university, school of basic and remedial studies funtua, nigeria 2department of biology, bayero university, kano. However, its reducing power was weaker than that of bht, which exhibited the strongest reducing power. Moreover, calycotome villosa extract showed strong reducing power. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Phytochemical study and evaluation of the antioxidant. Free radical scavenging activity from different extracts.
The reducing power of petroleum ether, ethyl acetate, acetone and hydrolysed extract of eichhornia crassipes mart. Free radical scavenging activity of some nigerian medicinal. Ferric to ferrous ion reduction at low ph causes a colored ferroustripyridyltriazine complex to form. Quantification of the antioxidant activity of plant extracts. To obtain the extracts the methodology previously described by michiels et al.
The results of this research showed that the reducing power. The reducing powers of ethanol, methanol, and acetone extracts were found to be significantly different p reducing power. Total phenolic, total flavonoid, tannin content, and. The inhibition of alphaamylase is an important therapeutic target in the regulation of postprandial increase of blood glucose in diabetic patients. Ferric reducing antioxidant power colorimetric assay protocol. The reducing power of the extract and the standard drugs increased with an increase in concentration though the plant extract had higher antioxidant activity than the bht and ascorbic acid used as reference drugs figure 1.
Comparative analysis of phenolics, flavonoids, and. The freezedried extracts thus obtained were dissolved in the respective solvents at the concentration of 1 mg1 ml and used for assessment of antioxidant capacity through various chemical assays. Extracts were evaluated for ferric reducing antioxidant power frap and 1,1diphenyl2picrylhydrazyl dpph radical scavenging activities in vitro. The reducing power assay is often used to evaluate the ability of an antioxidant to donate an electron which is an important mechanism of phenolic antioxidant action.
Much lower reducing power was assisted to methanol and acetone extracts of h. The reducing power of the phenolic compounds is determined by the frap method by means of a series of dilutions of the stock solutions of the various methanol extracts for wae, eae, bue and aqueous extract aqe in distilled water. Screening of in vitro antioxidant activity of methanolic. Zygophyllaceae and arthrophytum scoparium chenpodiacea, two. Chemicals and reagents all chemicals and reagents were of analytical grade quality purchased from sigma, merck, germany and himedia, bombay, india.
Materials and methods the leaves of epipremnum aureum were collected from the campus of haffkine institute for training, research and testing, parel. Ferric reducing antioxidant power assay in plant extract. The antioxidant activity of the plant extracts with the dpph radical scavenging and reducing power method, were in the order hymenocardia ekebergia salacia icacina dalbergia. The reducing power activity of the extract increased with the concentration of the extract. Reducing powers of plant extracts are given in fig. A comparative analysis of antioxidant potentials of. The ferric reducing ability of plasma frap as a measure. In the frap assay, mango peel extract at 200 ppm, guava peel extract at 400 ppm and papaya peel extract at 1200 ppm, exhibited reducing power comparable to the permissible amount of. Difference of solvent polarity to phytochemical content and. Methanolic extract of pulp and seed showed some degree of electron donation. Antioxidant properties of several medicinal plants growing. Pdf an investigation of the antioxidant property of. Halicacabum, a concentration of 100ugml has highest absorbance 1. Antioxidant and antimicrobial properties of five medicinal.
Reducing power assay rpa the reducing power assay rpa of the extracts was assessed as described by yildirim et al. Different parameters studied include phenolic contents, moisture, ash, crude fiber, fats and waxes. Phytochemical screening on the extracts revealed the presence of flavonoids, saponins, and tannins. The extracts of medicinal plants and natural products have become a great source of antioxidant and antiageing compounds sumazian. Various concentrations of pluchea leaves extract were mixed with phosphate buffer 200 mm ph 6,6 and potasium ferricyanide 0,1 %, and then solution was incubated at 50oc for 20 minutes. Pdf ferric reducing antioxidant power assay in plant extract. Freeradical scavenging capacity and reducing power of. Reducing power indicated in figures 3 and 4, total phenolic and fla the reducing capacity of the plant extract components may serve as a significant indicator of its potential antioxidant activity gulcin et al. The phytochemical screening of these extracts revealed the. Msse has been found to show better reducing capacity than the standard ascorbic acid.
Academic sciences asian journal of pharmaceutical and. Methanolic extracts of the plants were analyzed for their. Evaluation of dpph radical scavenging activity and. Screening of in vitro antioxidant activity of methanolic leaf.
Briefly, a set of 5 dilutions of each plant extract was prepared in 50% aqueous methanol ranging from 5 mgl to 25 mgl. The higher the absorbance of the reaction mixture, the higher would be the reducing power. How do you calculate the frap activity of any plant. The extract was found to be a potent iron chelator with ic50 66. Oxidative stress plays a significant role in the pathogenesis of metabolic syndrome including diabetes mellitus dm. Pdf ferric reducing antioxidant power assay in plant. Reducing power the reducing power was determined according to the method of oyaizu 25. Assessment of total phenolic content and antioxidant activity of ficus. Plant extracts containing high levels of antioxidants have a great reducing power of metal salts specifically, rubus fruticosus and hordeum vulgare and can be used at lower concentrations for the production of nanoparticles than the plant material or extract with a power weaker metal salt reducer n. Reducing power of eucalyptus oleosa leaf extracts and. The present study investigated the alphaamylase inhibitory and antioxidant potential of selected herbal drugs used in the. Preparation of plant extracts for antioxidant property analysis and total polyphenol content.
The effect of various plant extracts was measured on several important oxidation indicators. Free radical scavenging activity and reducing power of. In all tests, methanolic extract, which had the highest total phenolic. Reducing power the reducing power of the plant extracts was estimated by a basic method of oyaizu 9.
Many reports have revealed that there is a direct correlation between antioxidant activities and reducing power of certain plant extracts 26, 27. Reducing power was determined by taking different concentration of the plant extract. Three complementary assays, reducing power of fe iii, dpph radical scavenging and total antioxidant capacity were used for analysis of antioxidant properties of various extracts compared with the synthetic antioxidant, bht. A higher absorbance indicates a higher ferric reducing power. Assessment of antioxidant activity of plant extracts by different methods p. Plant materials guava fruits were harvested at maturity from one white. The reducing power activity of methanolic leaf and root extracts of h. Antioxidant and antidiabetic activities of the methanolic.
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